伪狂犬病病毒gN基因缺失株的构建及其在小鼠上的免疫效力分析

doi:10.11843/j.issn.0366-6964.2014.01.012

Abstract

Abstract:

The aim of this study was to determine the role of gN gene of pseudorabies virus (PRV). The PRVs rPRV-gE^-/TK^- and Bartha K61 were selected as parental strains, and mutants with gN gene deletion were constructed by homologous recombination. PCR amplification and sequence analysis showed that the mutants with gN gene deletion (rPRV-gE^-/TK^-/gN^- and rPRV-Ba-gN^-) were constructed successfully. Compared with parental strain, growth speed of rPRV-gE^-/TK^-/gN^- was slightly slow in the early stage and its titer was not affected on PK15 cells, while both growth speed and titer of rPRV-Ba-gN^-were significantly lower on PK 15 cells. The LD₅₀ of both strains rPRV-gE^-/TK^-/gN^- and rPRV-gE^-/TK^- were more than 1×10⁶TCID₅₀, the virulence of rPRV-Ba-gN^-was attenuated compared with the parental strain Bartha K61. The two gN deletion mutants could provide higher antibody level and protection against virulent PRV challenge than the parental strains. The deletion of gN gene in
PRV may contribute to decreased virulence and increased immune protection.

CLC Number:

S852.659.1

HU Yan-fen, GUAN Meng, LIU Kai-chun, CHEN Su-juan， PENG Da-xin， LIU Xiu-fan. Construction and Immune Response of Pseudorabies Virus Mutants with gN Gene Deletion[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(1): 87-93.

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Construction and Immune Response of Pseudorabies Virus Mutants with gN Gene Deletion

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